Unique Assays for Complex Scientific Questions

Incorporate complex 3D cell spheroid technology into your research.
While Likarda’s scientists frequently conduct cell-based studies in 2D, our expertise lies in developing complex 3D cell-based assays to answer your scientific questions. Our 3D assay technology uses a scaffold-free methodology. Our cell spheroids are uniform in size and run on 384-well plate formats. We’re able to produce them from a variety of different cell types in many different species whether primary cells or cell lines.

These assays can be useful for toxicology, metabolism, cell health and a number of other endpoints.

Unique Assay Development
New therapies that use the intact immune system, alter genes, or are comprised of cells as therapy require complex in vitro assays. Likarda’s scientists are experienced in developing and validating new assays that fit your needs. Outcome measures include questions about function, mechanism-of-action, and short- and long-term toxicity. Publications by our scientists highlight some of the unique capabilities of the team; however, most of our work is done for our clients and is unpublished or confidential.

Cell-Based Compound Screening
Likarda’s scientific team conducts large- and small-scale compound screens for academia, small start-ups, or large pharmaceutical companies. We utilize liquid handling robots and our patented CC MicroplatesTM to conduct high throughput screens in 2D or with 3D cell spheroids.

Our scientists have extensive experience in searching for synergies or antagonistic relationships between test compounds, as well as a variety of molecules including marketed drugs, environmental factors, or other test compounds. Our proprietary computer programs enable our robotics lab to test thousands of compound combinations in a short time frame with almost instantaneous results.

Cell Viability Assays
Cell viability assays are essential for answering a variety of your research questions. We routinely use colorimetric, luminescent, and fluorescent detectors of cell viability. And, for certain scientific goals, cell function may be a better representation of cell viability. Depending on your needs and goals, we use standard or custom assays, as well as develop novel cell viability tests.

Cytotoxicity Assays
We utilize a number of cell toxicity assays based on fluorescent, luminescent, or colorimetric read-outs. Alternatively, we have designed unique, multiplexed assays that measure cell toxicity, biomarkers, and cellular metabolic outcomes. These custom assays provide our clients with robust results at a lower cost and with less test compound and less time. Drs. Lindsey Ott and Karthik Ramachandran have published manuscripts on some of our novel assays.

Toxicity from drug-drug interactions is important information to obtain early in the drug development process. Likarda has proprietary programming that allows our liquid handling instruments to test for drug-drug interactions in a high-throughput manner, and (if needed) in 3D cell culture.

Drug-Drug Interactions
We have extensive experience in searching for synergies or antagonistic relationships between test compounds and a variety of molecules including marketed drugs, environmental factors, nutraceuticals, or other test compounds. Our proprietary computer programs enable our robotics lab to test thousands of compound combinations in a rapid time frame with almost instantaneous results. Likarda’s data have been included in multiple patents for our clients to support novel combination drug therapies.