Assay Development, Validation & Testing

Answer Complex Scientific Questions

Complex 3D Cell-Based Assays
While Likarda’s scientists frequently conduct cell-based studies in 2D, our expertise lies in developing complex 3D cell-based assays to answer your scientific questions. Our 3D assay technology uses a scaffold-free methodology. Our cell spheroids are uniform in size and run on 384-well plate formats. We’re able to produce them from a variety of different cell types in many different species whether primary cells or cell lines. These assays can be useful for toxicology, metabolism, cell health and a number of other endpoints.

Unique Assay Development
New therapies that use the intact immune system, alter genes, or are comprised of cells as therapy require complex in vitro assays. Likarda’s scientists are experienced in developing and validating new assays that fit your needs. Outcome measures include questions about function, mechanism-of-action, and short- and long-term toxicity.

Cell-Based Compound Screening
Likarda’s scientific team conducts large- and small-scale compound screens, using liquid handling robots and our patented CC MicroplatesTM to conduct high throughput screens in 2D or with 3D cell spheroids.

Our scientists have extensive experience in searching for synergies or antagonistic relationships between test compounds, as well as a variety of molecules, including marketed drugs, environmental factors, or other test compounds. Our proprietary computer programs enable our robotics lab to test thousands of compound combinations in a short time frame with near instantaneous results.

Cell Viability Assays
Cell viability assays are essential for answering research questions. We routinely use colorimetric, luminescent, and fluorescent detectors of cell viability. And, for certain scientific goals, cell function may be a better representation of cell viability. Depending on your needs and goals, we use standard or custom assays, as well as develop novel cell viability tests.

Cytotoxicity Assays
We use a number of cell toxicity assays based on fluorescent, luminescent, or colorimetric read-outs. Alternatively, we have designed unique, multiplexed assays that measure cell toxicity, biomarkers, and cellular metabolic outcomes. These custom assays provide robust results at a lower cost and with less test compound and less time.

Toxicity from drug-drug interactions is important information to obtain early in the drug development process. Likarda has proprietary programming that allows our liquid handling instruments to test for drug-drug interactions in a high-throughput manner, and (if needed) in 3D cell culture.

Drug-Drug Interactions
We have extensive experience in searching for synergies or antagonistic relationships between test compounds and a variety of molecules including marketed drugs, environmental factors, nutraceuticals, or other test compounds. Our proprietary computer programs enable our robotics lab to test thousands of compound combinations in a rapid time frame with almost instantaneous results. Likarda’s data have been included in multiple patents for our clients to support novel combination drug therapies.

Drug Discovery & Screening

Change and Save Lives

Drug discovery identifies potential new medicines and involves a wide range of scientific disciplines, including biology, chemistry and pharmacology.

With dedicated specialists who listen to your needs and a team of experts in screening, profiling, and custom assay development, Likarda can expand your research team by providing support before, during, and after your experiments. Our robotic approach to high-throughput 3D compound screening and optimization helps in early efficacy and toxicity screening along with formulation optimization.

In addition to the biological assays, we offer a full range of chemical analysis using mass spec and chromatography with expertise in the unique demands of plant-based components.

Spent Media Analysis

Change and Save Lives

Culture media and media changes can affect the growth conditions and productivity of cells during cell culture.  To ascertain performance, we perform Spent Media Analysis on media samples at varying time points to see how nutrient conditions change over time.  Our current analysis offers both amino acid and water soluble vitamin analysis.  Amino acid analysis includes the 20 most commonly found animo acids in cell culture media.  Water-soluble vitamin analysis includes niacin, thiamine, riboflavin, pantothenic acid, biotin, folic acid, pyridoxine, cobalamin, and ascorbic acid.  Analysis is completed by a HPLC with UV.

Analytical & Biological Services

Move Forward With Proof and Confidence

Confidently navigate a complex market with our multifaceted early-phase research tools and processes. Using our proprietary 3D screening approach, we test different drugs with known effects in humans using the conventional pharmaceutical approach and our 3D cell cluster. In each case, the results of our 3D approach better mimicked how the drugs work in humans. Compared to conventional methods, our process allows you to better predict responses to drug treatments.

Likarda has developed and worked with 3D models in cancer, diabetes, stem cells, liver, and a number of other areas.  We develop cell-based assays tailored to a specific cell type of interest, whether it is human, animal, cell lines or other source of origin.

While competitors can create and test 384 cell clusters in a normal cell culture plate, our micromold allows the formation and testing of 200,000 clusters in the same space. Our approach to 3D cell-based testing is highly efficient, allowing us to return findings with speed and accuracy.

We achieve accurate, high-quality results across all phases of drug discovery and development, including:

  • Toxicology
  • Metabolism
  • Cell health assays
  • Pharmacodynamics studies
  • Other cell-based assays
  • Preclinical animal studies
  • Mass spectrometry
  • Analytical chemistry

Analytical services are composed of comprehensive, state-of-the-art assay development, transfer, validation, and sample analysis.

Plus, we have a full suite of stability services, including environmental and photostability storage and testing that meet ICH guidelines. All our systems are constantly monitored to ensure performance and security and involve the following factors:

  • Cold storage (-80°C, -20°C, +4°C)
  • Room temperature
  • Environmental stability (controlled temp and RH)
  • Freeze/thaw
  • Photostability
  • Other stability conditions available upon request

Likarda also offers additional support services:

  • Review and interpretation of all study-related data
  • IND-enabling recommendations and studies
  • Preparation of peer-reviewed and expert opinion publications
  • Review of materials for brochures or collateral materials
  • Assistance or presentation of oral presentations for business or science audiences
  • Expedited services available (rapid turnaround times)
  • Likarda can offer GxP-compliant services

Accessory Services

Take Discoveries to the Next Level

Likarda’s research and analytic services are designed, and continue to evolve with science, in ways that empower you to achieve goals faster and more affordably than ever imagined. Our team, consisting of researchers and entrepreneurs, provides or assists with accessory services for a comprehensive source for getting discoveries to the next level.

Consultation
Our team includes academic scientists and engineers with expertise in cell biology, bioengineering, physiology and analytical chemistry, along with industry scientists from traditional pharmaceutical companies and immunization therapy. In addition, they have expansive experience in GLP/GMP environments. Depending on your needs, consultative services can help with:

  • Study designs
  • Understanding governmental regulations
  • Analysis of existing data either privately held or in the public domain
  • Formation of scientifically based marketing materials

Grant Writing
The most successful grant proposals are tailored to your specific needs and the funding program goals. At Likarda, we take pride in being able to identify and analyze grant opportunities. The Likarda team has garnered over $5 million in external grants for their own projects and those of their collaborators. We provide the following:

  • Extensive research
  • Need assessments
  • Program model development
  • Creation of management plans
  • Evaluation design
  • Budget planning
  • Completion of required federal and agency forms

Manuscripts, Patents and Presentations
The Likarda team has published more than 70 peer-reviewed scientific papers and, at any given time, is working on another 10–15 publications. We can assist you with preparing manuscripts and presentations for business or scientific audiences. We also can help review and prepare materials for patents and regulatory agencies.

Other services:

  • Review and interpretation of all study-related data
  • IND-enabling recommendations and studies
  • Preparation of peer-reviewed and expert opinion publications
  • Review of materials for brochures or collateral materials
  • Assistance or presentation of oral presentations for business or science audiences
  • Expedited services available (rapid turnaround times)

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