Microencapsulation and Cell Therapies

Core-Shell Spherification™: a shield and delivery option for all types of cell-based treatments

Cells are the building blocks of life. When living cells are medicine, they have the potential to treat or cure a wide variety of diseases and ailments. Current examples of cell therapies include bone marrow transplants, stem cell treatments, and islet transplants.  For many years, researchers and pharmaceutical companies have been investigating the use of cell-based treatments for some of our most debilitating diseases. Microencapsulation enables nutrients, proteins, small-molecules and other essential chemicals to freely diffuse through a hydrogel maintaining cell viability while holding them in a target location in the body. Alginate and alginate-based materials have been the most popular material for use because of desirable manufacturing characteristics. However, time and time again, no matter how many modifications are made to the alginate and its backbone, these formulations typically fail clinically.

Core-Shell Spherification™(CSS) is our patented formulation technology that microencapsulates cells and cell clusters using hydrogels that were once thought to be usable only in a bulk or large format. CSS is specifically designed for advanced hydrogels with slower gelation rates compared to alginate.  Examples include hyaluronic acid and PEG-based hydrogels. For the first time, it’s possible to produce microspheres with a wide variety of chemical, physical, and bioactive properties. Tuning the formulations allow control of microbead size, degradation rates, and cell loading densities, providing a wealth of opportunity to create successful cell-based therapeutics.

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 Encapsulated cell clusters

Developing novel therapies for pets or humans

Our work on developing novel therapies for pets suffering from endocrine diseases is just the beginning. Our proprietary technologies, including 3D cell culture and microencapsulation, plus our R&D expertise make us a great partner to support developing your cell-based therapy.

In addition to focusing on the application of canine diabetes, we are in the early stages of designing and testing cell therapies for the treatment of sepsis and inflammatory conditions such as arthritis.  In the case of arthritis, a specially-designed hydrogel formulation maintains the encapsulated stem cells in the afflicted joint for the sustained application of healing biomolecules.

Use our proprietary technologies for your cell therapies

Upcoming treatments in cell therapies include natural or engineered/genetically-modified cells (or medicine-producing cells) that secrete bioactive molecules that provide localized or systemic therapeutic effects. The ability to keep cells within a defined space in the body using our “sticky” microencapsulation formulation is an advantage over other microparticle administration procedures.

As you are developing your cell therapies, CSS can provide you the encapsulation that your cell therapy requires for successful injection and therapeutic effect.  Cell therapies present the opportunity to change the way we practice medicine and Likarda’s CSS technology opens new applications and opportunities that are otherwise unavailable.  Contact Likarda for development, partnering, or licensing opportunities.

Move confidently forward with your cell therapies

 Encapsulated cell clusters

Learn more: enter your information to download the the application note.

CC Microplates

Enabling production of a large number of uniform spheroids for drug discovery, research, and cell therapies.

Improving Miniaturized Organ-Like Cell Clusters

If a new, promising drug fails, it’s usually due to toxicity issues with organs. Predicting toxicity using 2D testing wastes valuable time and resources that could be spent on moving on to the next promising drug.

Three-dimensional cell clusters are not new to science. However, our proprietary and patented CC Microplate brings life-saving treatments to market faster and more affordably by producing 200,000 cell clusters in the same amount of space as other microplates produce only 384. That’s time and money saved.

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Produce 200,000 cells in our 384-well microplate

Each well has 150 microwells etched into the glass surface

Better Predication of Drug Efficacy

Cells are free to move, aggregate, and react as they would in a 3D environment for better prediction of a body’s response to tested drugs. Our 3D cluster technology allows cells to be maintained for a longer period of time, and it has been incorporated within standard well plate formats to offer a solution that can integrate with industry standard automated equipment. This efficiency gives Likarda or your lab an advantage over competitors by offering a truly scalable solution with vast applicability in:

  • Drug testing
  • Developing cell-based transplantations
  • Regenerative medicine

READ: Custom 3D Assays and Research Services Result in Better Predictability of Drug Treatment Response

Our CC MicroplatesTM also can be combined with currently available assays to screen for endpoints such as:

  • Viability
  • Function
  • Toxicity
  • Mechanism of Action
  • Protein Production

Benefits of Likarda’s CC MicroplateTM in your assay or lab:

  • The ability to form clusters from different tissues and populations: Age-, sex-, species-, or breed-specific testing
  • A low diffusion barrier ensures total drug exposure
  • Uniform cluster size
  • Averaged response from multiple clusters per well
  • Glass bottom enables high content microscopy
  • Improved prediction of native response by the body
  • Efficient scalability and SLAS-compliant for automated applications
  • Available now in a sterile, 384-well format

Glass bottom allows for imaging cell-based assays of 3D spheroids

Learn more: enter your information to download the the application note.