Microencapsulation

Core-Shell Spherification™: An Immunoprotective Shield for All Types of Cell-Based Treatments

When donor cells are transplanted into a recipient’s body without protection, the immune system attacks them as foreign. Traditionally, systemic immunosuppressive drugs are administered in order to prevent the attack and protect the cell transplant.

Instead, we microencapsulate (coat) our cells and cell clusters in a hydrogel material that blocks the immune system from attacking. Core-Shell Spherification™(CSS) is our patented process that microencapsulates cells and cell clusters using hydrogels that were once thought to be usable only in a bulk or large format.

These beads, ranging in size from 300-1000 microns, protect the encapsulated cells from destruction by the immune system without the need for immunosuppression drugs, while still allowing small molecules and nutrients (oxygen, glucose, insulin) to nourish the cells.

CSS is specifically designed for advanced hydrogels with much slower gelation rates compared to alginate. Examples include hyaluronic acid and PEG-based hydrogels. For the first time, it’s possible to produce microspheres with a wide variety of chemical, physical, bioactive properties.

CSS protects transplanted cells from:

  • Immune rejection
  • Loss of phenotype
  • Unwanted migration
  • Tumorigenicity (stem cells)

 Encapsulated cell clusters

Proprietary Technologies for Your Cell Therapies

Our work on developing novel therapies for pets suffering from endocrine diseases was just the beginning. Our proprietary technologies, including 3D cell culture and microencapsulation, plus our R&D expertise make us a great partner for developing your cell-based therapy.

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Cell Therapies

Developing Novel Cell Therapies for Pets and Humans

Cells are the building blocks of life. When living cells are medicine, they have the potential to treat or cure a wide variety of diseases and ailments. Current cell therapies include bone marrow transplants and stem cell treatments.

Our Kanslet™ transplantation system is a simple procedure that transplants insulin-producing cell clusters via injection into pets with diabetes to improve quality of life for them and their owners. Using our patented CC MicroplateTM, we produce scaffold-free, uniform cell clusters in a highly efficient, scalable way. Many cells prefer a three-dimensional format, better mimicking their natural environment to maintain normal function. These cell clusters can be made from most cell types.

READ: $4 Million Animal Health Investment Means More Strides for Treating Diabetic Pets

 Encapsulated cell clusters

CC Microplates

Developing Novel Cell Therapies for Pets and Humans

When donor cells are transplanted into a recipient’s body without protection, the immune system attacks them as foreign. Traditionally, systemic immunosuppressive drugs are administered in order to prevent the attack and protect the cell transplant.

Instead, we microencapsulate (coat) our cells and cell clusters in a hydrogel material that blocks the immune system from attacking. Core-Shell Spherification™(CSS) is our patented process that microencapsulates cells and cell clusters using hydrogels that were once thought to be usable only in a bulk or large format.

These beads, ranging in size from 300-1000 microns, protect the encapsulated cells from destruction by the immune system without the need for immunosuppression drugs, while still allowing small molecules and nutrients (oxygen, glucose, insulin) to nourish the cells.

Improving Miniaturized Organ-Like Cell Clusters

If a new, promising drug fails, it’s usually due to toxicity issues with organs. Predicting toxicity using 2D testing wastes valuable time and resources that could be spent on moving on to the next promising drug.

Three-dimensional cell clusters are not new to science. However, our proprietary and patented CC Microplate brings life-saving treatments to market faster and more affordably by producing 200,000 cell clusters in the same amount of space as other microplates produce only 384. That’s time and money saved.

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Produce 200,000 cells in our 384-well microplate

Each well has 150 microwells etched into the glass surface

Better Predication of Drug Efficacy

Cells are free to move, aggregate, and react as they would in a 3D environment for better prediction of a body’s response to tested drugs. Our 3D cluster technology allows cells to be maintained for a longer period of time, and it has been incorporated within standard well plate formats to offer a solution that can integrate with industry standard automated equipment. This efficiency gives Likarda or your lab an advantage over competitors by offering a truly scalable solution with vast applicability in:

  • Drug testing
  • Developing cell-based transplantations
  • Regenerative medicine

READ: Custom 3D Assays and Research Services Result in Better Predictability of Drug Treatment Response

Our CC MicroplatesTM also can be combined with currently available assays to screen for endpoints such as:

  • Viability
  • Function
  • Toxicity
  • Mechanism of Action
  • Protein Production

Benefits of Likarda’s CC MicroplateTM in your assay or lab:

  • The ability to form clusters from different tissues and populations: Age-, sex-, species-, or breed-specific testing
  • A low diffusion barrier ensures total drug exposure
  • Uniform cluster size
  • Averaged response from multiple clusters per well
  • Glass bottom enables high content microscopy
  • Improved prediction of native response by the body
  • Efficient scalability and SLAS-compliant for automated applications
  • Available now in a sterile, 384-well format

Glass bottom allows for imaging cell-based assays of 3D spheroids

Learn more: enter your information to download the the application note.